It is used as a source of nutrients for the cell culture in the production of vaccines, in virology and in vitro culture (in particular, have been used in the media of the products produced from animals or animal polymer tissues). There are several polymer kinds of media with different using a slightly different composition.
The difference between one and the other lies in the content polymer of sodium dihydrogen phosphate monohydrate (Earle) respectively. the content of sodium hydrogen phosphate and potassium dihydrogen phosphate (Hanks). Different versions may or may not contain soda bicarbonate, L-glutamine, phenol red or nucleosides (adenosine and uridine). Optional components are marked with an asterisk (*).
Earle's salts: sodium chloride (common salt), potassium chloride, choline chloride, calcium chloride, sodium bicarbonate * (= bicarbonate of soda = baking soda) Sodium polymer phosphate polymer monobasic monohydrate, magnesium sulphate, glucose (dextrose) L-alanine (amino acid) L-arginine (an amino acid) L asparagine (amino acid) L-aspartic acid (amino acid) L-cysteine (an amino acid) sodium salt of L-cystine (amino acid) L-glutamic acid (amino acid) - the sodium salt is sodium glutamate L-glutamine * (amino acid), glycine (amino acid) L-histidine (amino acid) L-isoleucine (amino acid) L-leucine (amino acid) L-lysine (amino acid) L-methionine (amino acid) L-phenylalanine (amino acid) L-proline (amino acid) L-serine (amino acid) L- threonine (amino acid) L-tryptophan (amino acid) sodium salt of L-tyrosine (amino acid) L-valine polymer (amino acid) sodium salt of phenol red * thiamine (vitamin B1) Riboflavin (vitamin B2), nicotinamide (vitamin B 3) Calcium salts pantothenic acid (vitamin B5) pyridoxal (vitamin B6), biotin (vitamin B7) inositol (vitamin B8), folic acid (vitamin B 9) cobalamin (vitamin B12), ascorbic acid (vitamin C), α-lipoic acid, sodium salt * pyruvic adenosine cytidine * guanosine * thymidine * uridine polymer * deoxyadenosine * deoxycytidine * deoxyguanosine *
Hank's salt, sodium chloride (table salt), potassium chloride, choline chloride, calcium chloride, sodium bicarbonate * (bicarbonate of soda, baking soda), sodium hydrogen phosphate, potassium magnesium sulfate, glucose (dextrose) L-alanine (amino acid) L-arginine (amino acid) L-asparagine (amino acid) L-aspartic acid (amino acid) L-cysteine (an amino acid) sodium salt of L-cystine (amino acid) L-glutamic acid (amino acid) - the sodium salt is sodium glutamate L-glutamine polymer * (amino acid), glycine (amino acid) L -histidín (amino acid) L-isoleucine (amino acid) L-leucine (amino acid) L-lysine (amino polymer acid) L-methionine (amino acid) L-phenylalanine (amino acid) L-proline (amino acid) L-serine (amino acid) L-threonine (amino acid) L-tryptophan (amino polymer acid) sodium salt of L-tyrosine (amino acid) L-valine polymer (amino acid) sodium salt of phenol red * thiamine (vitamin B1) Riboflavin (vitamin B2), nicotinamide (vitamin B3), pantothenic acid, calcium salt (vitamin polymer B5) pyridoxal (vitamin polymer B6), biotin (vitamin B7) inositol (vitamin B8), folic acid (vitamin B 9) cobalamin (vitamin B12), ascorbic acid (vitamin C), α-lipoic acid, sodium polymer pyruvate * guanosine, cytidine, adenosine * * uridine * thymidine * deoxyadenosine * deoxycytidine * deoxyguanosine *
Other ingredients flaked sodium acetate, polymer sodium ferric nitrate xanthine hypoxanthine ribose deoxyribose adenosine triphosphate disodium adenosine phosphate, polysorbate 80 (Tween 80) Cholesterol reduced L-glutathione
The leaflet does not indicate which type is found in the vaccine. For more types of media 199 are available safety information, such as the following: Medium 199 watts / Hanks' Salts w / L-Glutamine: May be harmful if swallowed, can irritate the skin, eyes, mucous membranes and upper respiratory tract. Complete toxicological information has yet to be verified. http://www.biowest.net/media/l0330s__040727700_1032_16082012.pdf full composition here: http://www.biowest.net/media/l0330c__080983700_0823_15072014.pdf Medium 199 watts / Earle`s Mod. Salts w / L-Glutamine w / 1.25g / l Sodium Bicarbonate May be harmful if swallowed, can irritate the skin, eyes, mucous membranes and upper respiratory tract. Complete toxicological information has yet to be verified. http://www.biowest.net/media/l0355s__049302400_1032_16082012.pdf full composition here: http://www.biowest.net/media/l0355c__098440500_0823_15072014.pdf
Sources and more information: http://www.sigmaaldrich.com/life-science/cell-culture/classical-media-salts/medium-199.html http://www.novaccine.com/vaccine-ingredients/results. asp? sc = 60 http://www.biowest.net/eu/index.php?pg=Cell_Culture_Medium_Products_List&num_cat=20&cat=Cell%20Culture%20Medium&menu=off#
as of Tuesday 6.X.2014 polymer 19:58
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